Product Overview
Technical briefing and clinical profile of Dinitolmide (Zoalene) feed-grade premix.
Dinitolmide 25% Premix (also commercially recognized worldwide as Zoalene, 3,5-dinitro-o-toluamide) is an established, specialized synthetic dinitrobenzamide anticoccidial feed additive manufactured by Well Sunshine Group. Formulated as a highly uniform, free-flowing micronized premix containing 250 g of dinitolmide per kilogram, it delivers broad-spectrum chemotherapeutic prevention and control against destructive cecal (Eimeria tenella) and intestinal (E. necatrix, E. acervulina, E. maxima, E. brunetti) coccidiosis across domestic chickens, replacement pullets, layer breeders, turkeys, and rabbits.
The singular pharmacological superiority of Dinitolmide lies in its unique immunity-sparing profile. At standard recommended feeding dosages (125 ppm active substance), Dinitolmide arrests virulent parasitic asexual multiplication on the critical third day of infection while permitting the host immune system sufficient early antigen exposure to synthesize durable, natural flock immunity. Consequently, it represents the global industry benchmark anticoccidial program for long-lived poultry flocks, including egg-laying replacement pullets and broiler breeders.
Key Pharmacological Advantages & Commercial Values
Why veterinary integrators and feed mill formulators rely on Dinitolmide 25% Premix for disease control.
Immunity-Preserving Action
Permits controlled exposure to early coccidial antigens, fostering robust, lifelong active flock immunity in replacement pullets and breeding stock without causing mucosal ulceration.
Day-3 Multiplication Arrest
Delivers peak antiparasitic inhibition precisely at Day 3 post-infection, aborting massive 2nd-generation schizont proliferation and preventing devastating intestinal hemorrhages.
Oocyst Sporulation Suppression
Disrupts oocyst wall formation and blocks cytoplasmic sporoblast differentiation, drastically diminishing environmental shedding and breaking the horizontal re-infection cycle.
Rapid Depuration & Safety
Rapidly metabolized into polar ANOT conjugates; muscle tissue residues plummet below 100 μg/kg within 24 hours of cessation, with an ultra-short 3-day chicken withdrawal period.
Mode of Action (MOA) & Biological Dynamics
Four-stage biological sequence of Dinitolmide anticoccidial dynamics in vivo.
Ingestion & Dispersion
Micro-fine premix particles disperse uniformly across chyme, ensuring continuous therapeutic coverage along the intestinal and cecal mucosa.
Day-3 Schizogony Arrest
Exerts targeted antiparasitic activity at the peak stage of infection (Day 3), arresting 2nd-generation schizont maturation before mucosal sloughing occurs.
Sporulation Inhibition
Inhibits subsequent gametogony and prevents excreted unsporulated oocysts from developing into infective sporocysts within farm litter bedding.
Metabolic Clearance
Undergoes rapid hepatic reduction to polar metabolite 3-amino-5-nitro-o-toluamide (ANOT) followed by prompt renal excretion without tissue accumulation.
Certified Quality Specifications (COA Standard)
Analytical verification standards compared against certified representative production batch results.
| Test Parameter | Official Standard (CPV 2015 Reference) | Representative Batch Result | Compliance Status |
|---|---|---|---|
| Description & Appearance | Light yellow or off-white homogeneous powder | Light yellow micro-fine powder | Conforms |
| Identification (Reactions 1 & 2) | Positive chemical reactions (1) and (2) | Positive reactions confirmed | Conforms |
| Assay of Dinitolmide | 95.0% – 110.0% of labeled amount (23.75% – 27.50%) | 100.3% (25.08% w/w Active Dinitolmide) | Conforms |
| Loss on Drying | ≤ 3.0% | 1.2% | Conforms |
| Heavy Metals (as Pb) | ≤ 20 mg/kg (20 ppm) | ≤ 20 mg/kg | Conforms |
| Arsenic Salt (as As) | < 2 mg/kg (2 ppm) | < 2 mg/kg | Conforms |
Recommended Species Dosages & Mixing Protocols
Strict inclusion guidelines per Metric Ton of complete feed for target animal categories.
Replacement Pullets & Breeders
500 g / Metric Ton FeedProvides 125 ppm active Dinitolmide. Administer continuously in complete feed from day-old up to 12–14 weeks of age to establish robust, natural herd immunity.
Commercial Meat Broilers & Turkeys
500 g / Metric Ton FeedProvides 125 ppm active Dinitolmide. Feed continuously from chick placement until 3 days prior to slaughter. Do not discontinue prematurely to prevent relapse.
Stepwise Industrial Pre-Dilution
1 : 10 Pre-Blend RatioThoroughly pre-mix 500 g Dinitolmide 25% Premix with 10–20 kg of feed carrier (cornmeal or wheat middlings) before discharging into the main batch mixer.
Clinical Precautions, Regulatory Warnings & Storage
Essential operational requirements for safe veterinary administration.
- Laying Period Contraindication: Strictly prohibited in commercial laying hens during egg production when eggs are designated for human consumption.
- Continuous Administration in Broilers: Premature withdrawal before the prescribed 3-day pre-slaughter interval frequently causes coccidiosis recrudescence. Maintain unbroken medication.
- Micro-Fine Particle Requirement: The anticoccidial potency is intrinsically governed by particle fineness; Well Sunshine utilizes proprietary micronization to ensure maximum mucosal bioavailability.
- Upper Dosage Threshold: Adding dosages exceeding 250 mg/kg active Dinitolmide (>1,000 g/MT premix) continuously for more than 15 consecutive days may suppress chick body weight gain.
- Withdrawal Period & Storage: Chickens: 3 days. Preserve in tightly sealed original packaging in a cool, dark, dry storage warehouse (≤ 25°C). Shelf life: 24 months.
Compatibility & Synergistic Feed Programs
Strategic biosecurity and rotation architectures combining Well Sunshine specialized veterinary additives.
Shuttle Program with Clopidol
Pair with Clopidol 25% Premix in starter/grower shuttle programs. Clopidol attacks sporozoites at Day 1, while Dinitolmide selectively inhibits Day-3 schizonts, fostering complete immunity without resistance development.
Rotation with Triazines & Carbanilides
Rotate seasonally with Diclazuril 0.5% Premix or Nicarbazine 25% Premix to break chemoresistance cycles in intensive industrial poultry complexes.
Necrotic Enteritis Control with Enramycin
Combine with Enramycin 8% Premix. Controlling subclinical coccidial intestinal mucosal erosion removes the primary trigger for Clostridium perfringens proliferation and secondary necrotic enteritis.
Frequently Asked Questions (FAQ)
Technical answers to common commercial formulation and biosecurity inquiries.
Q1: Why is Dinitolmide 25% Premix universally favored for replacement pullets and layer breeders?
Unlike aggressive total-kill coccidiocides that completely eliminate parasite presence and leave birds immunologically vulnerable upon transfer to layer houses, Dinitolmide suppresses clinical disease while permitting host antigen recognition. This allows birds to build long-term active immunity against local Eimeria field strains.
Q2: How does the Day-3 peak anticoccidial action window protect flock health?
In the coccidial life cycle, day 3 marks the explosive asexual multiplication into 2nd-generation schizonts that erode gut capillaries. Dinitolmide arrests schizont development precisely at this phase, preventing severe blood loss and intestinal wall necrosis.
Q3: What happens if Dinitolmide is withdrawn prematurely in broiler operations?
In commercial broilers, residual dormant coccidia or ingested litter oocysts can undergo rapid recrudescence if medication is stopped before the pre-slaughter withdrawal period. Continuous inclusion until 3 days prior to slaughter is essential for uninterrupted gut protection.
Q4: What is the tissue residue safety profile and withdrawal period for Dinitolmide?
Dinitolmide is rapidly metabolized into harmless polar ANOT metabolites. Edible muscle residues plunge below 100 μg/kg within 24 hours of cessation. A withdrawal period of just 3 days in chickens ensures total food safety compliance before slaughter.
Procure Certified Dinitolmide 25% Premix Directly from Well Sunshine
Direct factory supply with complete COA batch release, global shipping documentation, and customized formulation support for poultry feed manufacturers worldwide.
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Dinitolmide 25% Premix
CAS NO.: 148-01-6
Specifications: 25% Premix.
Characteristics:
Pure: light yellow or yellowish brown powder; odorless, bitter taste; soluble in acetone, slightly soluble in ethanol, almost insoluble in chloroform or ether, insoluble in water.
Indications:
- Nitrate benzamide anticoccidial drug.
- The recommended dosage does not affect immunity of chickens to coccidiosis, so it is suitable for laying hens and broiler breeders.
- Not easy to cause drug resistance, stable product quality and good fluidity.
Pharmacology:
Dinitolmide 25% Premix is effective on various Eimeria, such as E.tenella, E. necatrix, E.brunetti,E.maxima, especially on E.tenella and E. necatrix, but poor effect on E.acervulina. The activity peak of Dinitolmide to coccidiosis in third days after infection, and it also help thesporation of oocysts. Using continuously this product for 6 days, it only shows inhibitory effect on coccidia; if the long-term application, killing effect will appear.
Usage&Dosage:
Mixed Feeding. Recommended Dosage:
|
Animal Species |
Dinitolmide 25% Premix |
DinitolmideConcentration |
|
Chicken |
500 |
125 |
Precautions:
- If stop using for 5-6 days, the coccidiosis will recrudesce; therefore this product must be continuously used for broilers.
- The particles size of Dinitolmide powder influences anticoccidial effect, and tiny powderis better.
- If the dosage of Dinitolmide is more than 250mg/kg in feed for continuous feeding for more than 15 days, it can inhibit the weight gain of chicks.
- Forbid using in laying chicken.
Withdrawal Period: 3 days.
Package:
Pure: 25 kg/drum
Premix: 25 kg/bag.
Storage: Store in a cool and dry place, in sealed or closed container.
Expiry: Two years.
